Phase imaging

Cells are transparent, and consequently, are difficult to observe under the microscope. Lots of work has been done in order to produce contrast inside cells to be able to observe them. One approach is to use the phase shift of the light provoked by the cells themselves to give this contrast. In the phase contrast microscopy, the phase shift is translated into intensity variations. Each component will have a different intensity variation and thus a different intensity. However, as they are all mixed together, it is difficult to extract any quantitative information. The result can be translated into a topographic map of the cell although, there is no information on the different organelles’ 3D location.

However, thanks to its laser inclined at 45°, Nanolive’s new microscope, the 3D Cell Explorer, is able to convert this phase shift information into comprehensive data. The localization of each cell component can therefore be determined and distinguished, making it possible to extract quantitative data.

Nanolive’s technology allows you to:

  • Exceed the limits of the traditional phase contrast microscopy
  • Obtain a tomography of your samples, not only a superficial view (topography)
  • Have a complete 3D reconstruction of the internal compartments of living cells

In the left panel you have the usual results of a phase imaging acquisition. The representation of the cells is a superposition of all the intensity of the pixels, ranging from violet (low intensity) to yellow (high intensity). No information about the location of the cell’s interior substructures are given by this method. In the right panel, you have the reconstruction of the image with our software STEVE. You can appreciate how well the different structures of the cells are reconstructed, giving you all the information necessary about their location.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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