High confluency

On this page you will have the possibility to explore the cell culture with high confluency as we see it: in 3D and stain free! Enjoy and keep tuned: the list of results grows every week…

Nanolive’s technology allows for:
• Cellular multi-layer construction visualization
• Contact inhibition studies

In cell culture, confluence is the term commonly used as an estimate of the number of adherent cells in a culture dish or a flask, referring to the proportion of the surface which is covered by cells. For example, 100% confluency means the surface is completely covered by the cells, and no more room is left for the cells to grow as a monolayer. Many cell lines exhibit differences in growth rate or gene expression depending on the degree of confluence. Cells are typically passaged before becoming fully confluent in order to maintain their proliferative phenotype. Some cell types, not limited by contact inhibition, such as immortalized cells, may continue to divide and form layers on top of the parent cells. To achieve optimal and consistent results, experiments are usually performed using cells at a particular confluence, depending on the cell type.

The 3D Cell Explorer allows the acquisition of 3D images of your cell cultures in real-time and in a non-invasive way.

Lung cancer cells 100% confluency 

Human lung cancer cells were grown to 100% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). 

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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Related topics

Discover more about co-colture in our blog post “Amoeba and mouse cancer cells” “

Extend your cell culture over the 2D limit: 3D cell culture – HeLa cells embedded in alginate beads 

Beyond the contact inhibition, look the multilayer cell cultures in 3D

How it all began: low confluent cell colture. ‘Low confluency application page

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