A co-culture is a cell cultivation set-up in which two or more different populations of cells are grown with some degree of contact between them. Co-culture systems have long been used to study the interactions between cell populations and are fundamental in cell–cell interaction studies of any kind. The 3D Cell Explorer allows for the first time a real-time and non-invasive long-term co-culture monitoring. The microscope detects the RI (Refractive Index) of each cell allowing an automatic segmentation of cell populations. Moreover, Nanolive’s technology gives the possibility to study the cell interactions in 3D, allowing for XYZ measures with unprecedented nanometer resolution (x,y= 200nm; z=400nm).
B16 Mouse Melanoma cancer cells and Dictyostelium amoeba co-culture
Mouse skin melanoma cancer cells (B16, p35) were incubated overnight with dictyostelium amoebae cells (WT1, p14), in order to visualize their interactions. Our digital staining panel allows the discrimination of mammalian cells (blue) and amoeba cells (green) based on their specific refractive index (RI) range. The RI difference (ΔRI 0.015) allows for an automatic segmentation of cell populations. We observed a simultaneous, massive mammalian cell necrosis, the reason of which remains unknown.
The 3D Cell Explorer zoom-in capabilities and the XYZ measures of amoeba details.
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