Cancer cells and amoeba in co-culture

A co-culture is a cell cultivation set-up in which two or more different populations of cells are grown with some degree of contact between them. Co-culture systems have long been used to study the interactions between cell populations and are fundamental in cell–cell interaction studies of any kind.

In this blog post we want to show an example of a co-culture experiment performed using Nanolive’s 3D Cell Explorer. Mouse skin melanoma cancer cells (B16) were incubated overnight with dictyostelium amoebae cells (WT1), in order to visualize their interactions.  

Our digital staining panel allows the discrimination of mammalian (blue and violet) and amoeba cells (green) based on their specific refractive index (RI) range. The d-stain remains robust and consistent among several acquisitions and during the whole time-lapse (8h) allowing an automatic segmentation of cell populations.

We observed a simultaneous, massive mammalian cell necrosis, the reason of which remains unknown.

Enjoy, in 3D and and Real-Time, their explosion and release of internal components! We are always interested in your ideas and professional experience, don’t hesitate to share with the bioteam your comments and suggestions about a possible explanation of the biological phenomena.

Mouse skin melanoma cancer cells (B16, p35) were grown to 40% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). They were incubated overnight with dictyostelium amoebae cells (WT1, p14) grown in HL-5 medium. The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 8 hours, capturing images every minute.

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